Acessar o Zé Moleza
ou
O que você está pesquisando?
Disease-associated mutations in the prion protein impair laminin-induced process outgrowth and survival
713 visualizações
8327 palavras
Adicionar aos favoritos
Loading...- | |
the increment in Ca2+ levels did not achieve the threshold necessary to promote this
phenotype in these cells. It is important to
note that the 177N and 179I mutants are in the vicinity of the Ln γ1 binding site on the PrPC molecule, amino acids 173-182 (22). Thus, these alterations may abrogate the interaction between PrPC and laminin. On the other hand, we show similar results with PrPC molecules carrying disease-association mutations in the amino-terminal domain (101L, 104L, and 116V) or in the vicinity of the third α-helix region (199K). It is possible that these mutations instead affect the interaction between PrPC and mGluR1/5; however, the binding site between these molecules has not yet been identified.
PrPC was also pointed to protect neurons against injury both in vitro and in vivo (13; 19; 25). PrPC protein is itself a
receptor for the secreted form of STI1, an interaction which has been shown to
promote neuroprotection and neuronal differentiation (25, 68, 69). Our present
data indicate that Ln γ1 peptide impaired staurosporine-induced cell death in PrP3F4 cells while Ln γ1 peptide fails to rescue CF10 and CF10 cells expressing PrPC mutants from death. Indeed, the results are consistent with the participation of the Ln
rescues staurosporine-induced cell death. This seems to show the absence of a dominant-negative effect, which is consistent with previous data showing that neurodegeneration mediated by expression of PrPC deletion mutants (some…
phenotype in these cells. It is important to
note that the 177N and 179I mutants are in the vicinity of the Ln γ1 binding site on the PrPC molecule, amino acids 173-182 (22). Thus, these alterations may abrogate the interaction between PrPC and laminin. On the other hand, we show similar results with PrPC molecules carrying disease-association mutations in the amino-terminal domain (101L, 104L, and 116V) or in the vicinity of the third α-helix region (199K). It is possible that these mutations instead affect the interaction between PrPC and mGluR1/5; however, the binding site between these molecules has not yet been identified.
PrPC was also pointed to protect neurons against injury both in vitro and in vivo (13; 19; 25). PrPC protein is itself a
receptor for the secreted form of STI1, an interaction which has been shown to
promote neuroprotection and neuronal differentiation (25, 68, 69). Our present
data indicate that Ln γ1 peptide impaired staurosporine-induced cell death in PrP3F4 cells while Ln γ1 peptide fails to rescue CF10 and CF10 cells expressing PrPC mutants from death. Indeed, the results are consistent with the participation of the Ln
rescues staurosporine-induced cell death. This seems to show the absence of a dominant-negative effect, which is consistent with previous data showing that neurodegeneration mediated by expression of PrPC deletion mutants (some…
É esse o conteúdo que você precisa?
Faça seu login e saiba como ver o trabalho completo
Institucional
Saiba como fazer
Trabalhos Acadêmicos
O Zé Moleza facilita sua vida acadêmica ajudando você em suas pesquisas, e a economizar o seu tempo e o seu dinheiro nos seus trabalhos de faculdade. São mais de 26144 pesquisa acadêmicas entre elas, monografia, temas de monografias, TCC, modelos de monografias, trabalhos de universidades, resenha, Paper, Ensaio, Bibliografia, Trabalhos Escolares.
Dicas de como fazer: Capa de Monografia, capa de TCC, Regras da ABNT, como fazer monografia, como fazer Projeto Final, como fazer seminário, como fazer capas, referências bibliográficas, modelo de monografia.
O Zé Moleza NÃO faz a venda de monografia e É TOTALMENTE CONTRA a compra de monografia pronta e trabalhos prontos. O Zé Moleza NÃO auxilia a quem compra monografia, NÃO apóia a quem quer comprar Trabalhos Prontos, e NÃO APROVA a quem quer comprar TCC prontos, dando dicas de formatação, regras da ABNT, dando sugestões de temas para monografia, resumo de livros, projeto de pesquisa, projeto de mestrado, projeto de pós-graduação, trabalhos acadêmicos, incentivando o usuário a desenvolver por conta própria sua monografia.
